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Compound: DOPA (3,4-dihydroxy-L-phenylalanine)
Neurotransmitters |
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Application Notes: The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead a combination of ion-exchange and reversed-phase interactions were used. Application Analytes: Application Detection: |
Catecholamine Pathway |
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Application Notes: Primesep 200 separates catecholamines in the catecholamine pathway in 10 minutes. Phenylalanine, tyrosine, DOPA, dopamine, norepinephrine, and epinephrine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Application Detection: |
Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1) |
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Application Notes: Application Analytes: Application Detection: |
HPLC Separation of Compounds of Catecholamine Pathway |
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Application Notes: The catecholamine neurotransmitters are amino acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline resolved on a Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), trifluoracetic acid (TFA) or ammonium formate and ultraviolet (UV) detection at 210 nm. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase. Application Analytes: Application Detection: |
HPLC Separation of Polar and Hydrophobic Drugs on Obelisc R and N |
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Application Notes: Application Analytes: Application Detection: |
Separation of Serotonin, Dopamine, and Related Compounds |
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Application Notes: Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. Method is very sensitive to amount of ACN, buffer and buffer pH. Retention time changes with variation of main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.). Application Analytes:
Application Detection: |
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Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007