Compound: Betaine

Retention Characteristics of Betaine in Mixed-Mode HPLC

Application Notes:

The retention of the zwitterion betaine on Primesep 200 demonstrates the tunability of Primesep columns. Retention of betaine is altered by acid type, amount of acid in the mobile phase, and the amount of organic solvent. Typical reversed-phase columns do not show this tunability with simple mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

Application Analytes:

Application Detection:

HILIC Separation of Carboxymethylcysteine and Betaine

Application Notes:

In this HPLC method, betaine and carboxymethylcysteine are separated by HILIC mixed-mode mechanism. Betaine and carboxymethylcysteine are very hydrophilic compounds which are not retained on reverse phase columns. Compounds are separated by combination of HILIC and ion-exchange mechanisms. In HIILIC mode, Obelisc N columns allow operating at lower organic concentration for improved solubility of analytes. Method can be used for quantitative or qualitative analysis of betaine and carboxymethylcysteine using ESLD or LC/MS detection. Betaine in chemistry is any neutral chemical compound with a positively charged cationic functional group such as ammonium ion or phosphonium ion (generally: onium ions) which bears no hydrogen atom and with a negatively charged functional group such as a carboxylate group which may not be adjacent to the cationic site. Historically the term was reserved for tetramethylglycine only.

Application Analytes:

Application Detection:

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